![]() false-negatives / culture negative infections: ( see biofilm formation) some surgeons require that a sample yield 5 colonies per plate in order to indicate infection when cultures are taken at time of revision, surgeon should be suspicious if only one sample (out of many) grows a pathogen (generallyĢ positive culture samples are required to indicate a definite infection) cultures which show growth in broth only are generally not considered positive (probable lab error) Diagnosing Periprosthetic Infection: False-positive Intraoperative Gram Stains ![]() The role of intraoperative gram stain in the diagnosis of infection during revision total hip arthroplasty. The role of intraoperative Gram stain in revision total joint arthroplasty. Gram stain detection of infection during revision arthroplasty gram negative bacilli:, gram negative cocci:, gram positive bacilli:, gram positive cocci the sensitivity of intraoperative gram stain is only 27% be aware that the quality of the gram stain result depends to a degree on the quality of the technician performing the test some authors recommend not getting intraoperative gram stains. The natural progression of synovial fluid white blood-cell counts and the percentage of polymorphonuclear cells after primary total knee arthroplasty: a multicenter study. Synovial fluid white cell and differential count in the diagnosis or exclusion of prosthetic joint infection Gout-induced arthropathy after total knee arthroplasty: a report of two cases Acute crystal-induced arthritis following arthroplasty Analysis of Synovial Fluid in Culture-negative Samples of Suspicious Periprosthetic Infections Letter: Cell Count and Differential of Aspirated Fluid in the Diagnosis of Infection at the Site of Total Knee Arthroplasty Cell Count and Differential of Aspirated Fluid in the Diagnosis of Infection at the Site of Total Knee Arthroplasty Determining “True” Leukocytosis in Bloody Joint Aspiration Preoperative testing for sepsis before revision total knee arthroplasty. Synovial fluid leukocyte count and differential for the diagnosis of prosthetic knee infection. diff dx: gout and pseudogout synovitis w/p arthroplasty white cell count greater than 2,000/mm may indicate sepsis, when the majority of cells are PMNs most accurate method of dx, and is required prior to all revisions aspiration: gram stain, cell count & culture: Inflammatory Blood Laboratory Levels as Markers of Prosthetic Joint Infection: a Systematic Review and Meta-Analysis A Simple, Cost-Effective Screening Protocol to Rule Out Periprosthetic Infection Use of Erythrocyte Sedimentation Rate and C-Reactive Protein Level to Diagnose Infection Before Revision Total Knee Arthroplasty. C reactive protein and skin temperature post total knee replacement. Coventry Award: Synovial C-reactive Protein: A Prospective Evaluation of a Molecular Marker for Periprosthetic Knee Joint Infection
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